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Archive for April, 2012

IDENTIFIKASI PIGMEN BETASIANIN PADA BEBERAPA JENIS INFLORESCENCE Celosia

09 Apr

Retno Mastuti1), Yizhong Cai2) dan Harold Corke2)
1)Jurusan Biologi Fakultas MIPA UB; email: rmastuti@yahoo.com
2)School of Biological Science, The University of Hong Kong

sumber : limnologi.lipi.go.id/…/58_Identifikasi_Pigmen_Betasianin_Pada_Beb…

 
 

Production of intergeneric hybrid calli from C-3 and C-4 species of Amaranthaceae through protoplast fusion

04 Apr

by R Mastuti, H Miyake, T Taniguchi, Y Takeoka (full text pdf)

Japanese Journal of Crop Science (1997)
Volume: 66, Issue: 3, Pages: 456-465

Abstract

Intergeneric somatic hybrid calli were obtained through electrofusion between protoplasts of Celosia cristata L. (C-3 species) cell suspension and Amaranthus tricolor L. (C-4 species) cotyledon. The highest binary fusion frequency, 4 similar to 5%, was attained when the protoplasts were suspended in fusion solution containing 0.4 M sorbitol and 2.0 mM CaCl2, aligned by an AC held (200 V/cm, 0.5 MHz) for 30 s and fused by a single DC square pulse at 1.6 kV/cm for 40 mu s. Fusion products were identified by characteristics of both parents in the hybrid cells:they possessed green chloroplasts or pink plastids from cotyledonary protoplasts as well as a cytoplasm- rich colorless sector derived from cell suspension protoplasts. The occurence of two types of calli, compact and friable, obtained after culturing on proliferation medium lead to the isolation of putative hybrid calli without any artificial selection because C. cristata protoplasts regenerated only friable calli and A. tricolor protoplasts did not produce calli. A total of 14 lines of putative hybrid compact calli were obtained by screening on the basis of callus morphology. An isozyme analysis of acid phosphatase confirmed the hybridity of 14 lines of compact calli. Callus morphology was also studied by scanning electron microscopy.
 
 

ISOLATION AND CULTURE OF Celosia cristata L. CELL SUSPENSION PROTOPLASTS

04 Apr

Retno Mastuti*), Hiroshi Miyake**), Takeshi Taniguchi**), Yoji Takeoka**)
*) Biology Department, FMIPA, Brawijaya University, Jl. Veteran Malang, 65142
**) School of Agricultural Sciences, Nagoya University, Chikusa-ku 464-01, Japan

http://www.berkalahayati.org/index.php/bph/article/view/154/101